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Titlebook: Biomembrane Protocols; II. Architecture and John M. Graham,Joan A. Higgins Book 1994 Springer Science+Business Media New York 1994

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期刊全称Biomembrane Protocols
期刊简称II. Architecture and
影响因子2023John M. Graham,Joan A. Higgins
视频video
学科分类Methods in Molecular Biology
图书封面Titlebook: Biomembrane Protocols; II. Architecture and John M. Graham,Joan A. Higgins Book 1994 Springer Science+Business Media New York 1994
影响因子There have been many important advances in our understanding of biological membrane structure and function over the last decade. Much of this progress has been driven by the development of new techniques for studying membrane components and their interactions. Tradition­ ally, the investigation of membranes has largely occurred within the domains of biochemistry, physical chemistry, and cell biology; but many of the most significant advances have resulted from an expansion into other disciplines, such as molecular biology, immunology, and the clinical sciences. In these two volumes we have attempted to bring together some of these techniques—to combine the old and the new. Biomembrane Protocols: II. Architecture and Function is concerned exclusively with the architecture and activities of membranes, whereas the companion volume, Biomembrane Protocols: I. Isolation and Analysis, deals with their isolation and compositional analysis. The aim of each chapter is to provide detailed technical and meth­ odological information that will allow the reader to perform the tech­ nique successfully, without the need to consult other texts. Detailed reviews of membrane structure and function are
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Gerd Lutze,Alfred Schultz,Karin Luzi detergent to be removed following purification. Ionic detergents, such as cholate, usually fall into this category, although the nonionic detergent octylglucoside also has a high critical micelle concentration. Nonionic detergents, such as Triton X-100 and C.E., cannot be removed by conventional di
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Crystallization of Membrane Proteins for X-Ray Analysis,tudy of bacteriorhodopsin has the resolution of the structural analysis approached that obtainable by X-ray crystallography (.). The formation of two-dimensional crystalline arrays will not be considered in this chapter. The first membrane protein crystal structure, the bacterial photosynthetic reac
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Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spe been presented (.,.). From such data, straight-line plots of the mobile: immobile lipid ratio against lipid:protein ratio are usually constructed, with the intercept on the lipid:protein ratio axis being taken to be the number of lipid-binding sites on the protein and the slope giving the relative
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