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Titlebook: Biomedical Applications of Immobilized Enzymes and Proteins; Volume 1 Thomas Ming Swi Chang (Professor of Physiology, Pr Book 1977 Plenum P

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期刊全称Biomedical Applications of Immobilized Enzymes and Proteins
期刊简称Volume 1
影响因子2023Thomas Ming Swi Chang (Professor of Physiology, Pr
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图书封面Titlebook: Biomedical Applications of Immobilized Enzymes and Proteins; Volume 1 Thomas Ming Swi Chang (Professor of Physiology, Pr Book 1977 Plenum P
影响因子I) ADSORPTION EEEEEEEE E E carrier 2) COVALENT LINKAGE a) Insoluble support b) Intermolecular linkage N‘E~ ~~ c) Soluble support 0 :)....m 3) tM TRIX (MOLECULAR) ENTRAPMENT ~~~~~;;..,J~-polymer matrix 4) ENCAPSULATION membrane FIGURE I. Classification of immobilized enzymes. Covalently linked, adsorbed, and matrix-entrapped enzymes represent stage II, research on the microenvironment. Microencapsulation represents stage III, research on the intracellular environment. Further subdivision of microencapsulated enzymes will be found in Chapter 12. 4 T. M. S. CHANG matrix entrapment. In this section, detailed discussions will center on clinical analysis, urine analysis, monitoring of environmental pollution, radioimmune assay, enzyme-linked immunosorbent assay, enzyme electrodes, and other approaches involving immobilized enzymes, antibodies, and antigens. In the final section, research workers describe and discuss the perspectives of immobilized enzymes and proteins. Here, they speculate on the future potential of possible approaches, even though these may not have been extensively studied or tested at the laboratory stage. The biomedical applications of enzymes and proteins, especiall
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Covalent Linkage: III. Immobilization of Enzymes by Intermolecular Cross-Linking can produce enzyme conjugates of diverse physical character depending on the conditions employed. Thus, conjugates can be prepared that are water-soluble or water-insoluble; granular, gel-like, or filamentous in nature; or that are pure protein. It is reliable and inexpensive.
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-Asparaginase as a Model for Enzyme Therapy of Substrate-Dependent Tumorsy. Certain tumor cells, unlike normal cells, cannot synthesize enough asparagine and depend on extracellular supplies for asparagine. Selective depletion of this extracellular source of asparagine by .-asparaginase can lead to the suppression of asparagine-dependent tumors.
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Radzhana Buyantueva,Maryna Shevtsovaimmobilized systems of intracellular organelles or intracellular membrane systems. Enzyme and protein research and its application have gone through interesting stages (Table 1). Stage I included extensive research into the extraction, purification, and crystallization of enzymes from biological cel
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Thorsten Bonacker,Kerstin Zimmer immobilization can be achieved by intramolecular cross-linking reactions. However, since most of the techniques and reagents used for intermolecular cross-link formation are the same as those used to form intramolecular bonds, it is safe to conclude that intramolecular linkages are natural side rea
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Katie M. Barrow,Katherine R. Allenssary to use such methods and chemicals that the functional tertiary structure will not be affected. Physical entrapment of an enzyme in a gel lattice is an immobilization method in which no modification of the amino acid residues is needed, and which offers the advantage of reaction conditions usua
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