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Titlebook: Biomass Conversion; Methods and Protocol Michael E. Himmel Book 2012 Springer Science+Business Media, LLC 2012 Biomass Feedstocks.Cellulose

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Immunological Approaches to Plant Cell Wall and Biomass Characterization: Immunolocalization of Glycmonoclonal antibodies can be used to examine the distribution of plant cell wall glycan epitopes at the whole plant, tissue, cell, and subcellular levels. Understanding the basic cell wall structure is essential for devising efficient strategies to convert cell walls to fermentable sugars for ethanol production.
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A Method to Evaluate Biomass Accessibility in Wet State Based on Thermoporometryd on DSC isothermal step procedure is explained in details. This detects the amount of nonfreezing bound water in a wet sample and the value is expressed into pore size distribution with the Gibbs–Thomson equation. Information on pore size distribution and pore volume can be used to evaluate biomass accessibility.
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Bioprospecting Metagenomics for New Glycoside Hydrolaseschapter is to describe strategies, based on metagenomic approaches, for the discovery of glycoside hydrolases (GHases) from microbial biomass decay communities, especially those from unknown or never-been-cultivated microorganisms.
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Challenges for Assessing the Performance of Biomass Degrading Biocatalysts,but can also present pitfalls regarding experimental accuracy and relevance. Recent work demonstrates that HTP systems which include biomass composition analysis, thermal chemical pretreatment, and biomass saccharification can be realized.
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A Simple Method for Determining Specificity of Carbohydrate-Binding Modules for Purified and Crude Iharides, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) for determining the distribution of the CBM between the bound and unbound fractions. This technique enables qualitative assessment of the binding strength and ligand specificity for each CBM.
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Affinity Electrophoresis as a Method for Determining Substrate-Binding Specificity of Carbohydrate-Ar in some cases, enzymes are able to bind polysaccharides targets other than their specified substrate, and this knowledge can be essential to understand the basics of the intrinsic mechanism of these enzymes in their natural environment.
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https://doi.org/10.1007/978-3-663-16320-6(i.e., lignin and cellulose). This method can also be used to real-time monitor the chemical processes involved in biomass pretreatment. These together demonstrate CRS as an effective method for imaging complex chemistry in biological systems.
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