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Titlebook: Bioluminescence; Methods and Protocol Preston B. Rich,Christelle Douillet Book 2009Latest edition Humana Press 2009 ATP.Calcium.Colon.Expre

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发表于 2025-3-21 19:47:59 | 显示全部楼层 |阅读模式
期刊全称Bioluminescence
期刊简称Methods and Protocol
影响因子2023Preston B. Rich,Christelle Douillet
视频video
发行地址Provides an easily accessible reference volume for a wide range of bioluminescence assays and applications written by leading researchers in the field.Comprehensive guide to developing, validating, ru
学科分类Methods in Molecular Biology
图书封面Titlebook: Bioluminescence; Methods and Protocol Preston B. Rich,Christelle Douillet Book 2009Latest edition Humana Press 2009 ATP.Calcium.Colon.Expre
影响因子Part I I wasraised in a redbrick Baltimore row housewhere summer was marked by the ti- honored ritual of firefly-chasing – a backyard tradition that has endured the gene- tions.Amidtheexcitement,myfatheroftentoldthestoryofhow,whenhewasachild, researchers at the Johns Hopkins University had appealed for the systematic capture of live fireflies en masse. Science had engaged the Baltimore youth in an entrepreneurial quest to jar as many lightning bugs as the dwindling light of dusk would permit. The very next morning, each 100-count glass jar of glowing crawling insects could be exchanged at the University for exactly one crisp dollar bill. Unrecognized at the time by my father, his joyous endeavors had contributed in a profoundwaytotheadvancedmolecularbiologicaltechniquesthatserveasthebasisfor thistextbook.In1947,WilliamMcElroyusedextractsfromthoseveryfirefliestodefine the fundamental reaction underlying the mystical phenomenon of luminescence, and published ‘‘The energy source for bioluminescence in an isolated system’’ in the Proceedings of the National Academy of Sciences. Inthedecadessincethatsummer,thestudyandapplicationofbioluminescencehave allowed us to leverage the enduring p
Pindex Book 2009Latest edition
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书目名称Bioluminescence影响因子(影响力)




书目名称Bioluminescence影响因子(影响力)学科排名




书目名称Bioluminescence网络公开度




书目名称Bioluminescence网络公开度学科排名




书目名称Bioluminescence被引频次




书目名称Bioluminescence被引频次学科排名




书目名称Bioluminescence年度引用




书目名称Bioluminescence年度引用学科排名




书目名称Bioluminescence读者反馈




书目名称Bioluminescence读者反馈学科排名




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发表于 2025-3-21 20:18:56 | 显示全部楼层
Aufbau der Arbeit und Vorgehensweisejugating (either chemically or by molecular biology techniques) bioluminescent probes to specific targets, it is in fact possible to track a wide range of events and analytes. To aid investigators in the choice and applications of reporter genes, the materials and methods required for BL measurements and experimental protocols are described.
发表于 2025-3-22 01:39:06 | 显示全部楼层
Arbeitsdefinition von Komplexitätct interpretation of BLI results and validate BLI techniques. This chapter summarizes the use of calibrated light-emitting standards, bioluminescence tomography, and post-mortem validation of luciferase expression for validating quantitative BLI measurements.
发表于 2025-3-22 04:33:48 | 显示全部楼层
Aufbau der Arbeit und Vorgehensweisesophisticated questions of molecular biology by including specific promoter sequences. This chapter will describe routine methods used to support multiple investigators in our small animal imaging resource.
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Günther Schuh,Hans-Peter Wiendahltrate sequence of caspase-3 (Asp-Glu-Val-Asp). When activated caspase-3 digests the substrate sequence, the cyclized luciferase recovers its activity. The indicator provides a general means of evaluating effects of cytotoxic compounds or novel pharmacological chemicals and apoptosis.
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,Schlußbetrachtungen und Ausblick,er protein (PIN-G), containing HA, cMyc and GFP epitope, and fluorescence tags. Although originally designed for trafficking studies, pIN technology is a powerful tool applicable to almost every area of biology. Here we describe the methodologies used routinely in analyzing pIN constructs and some of their derivatives.
发表于 2025-3-23 07:17:03 | 显示全部楼层
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