Titlebook: Bioluminescence; Methods and Protocol Sung-Bae Kim Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive

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https://doi.org/10.1007/978-3-531-91181-6monstrates how to image RA activities indirectly in mammalian cells with ligand-activatable single-chain bioluminescence (BL) probes. We introduce 13 different molecular designs for characterizing an efficient single-chain probe that quantitatively visualizes RA activities with significant sensitivi

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Clare Woolhouse,Laura J. Nicholsoninase cascades are of high importance. Our group has developed a split-luciferase biosensor system to monitor the activity of the Hippo pathway, a kinase cascade that regulates a wide variety of cellular processes. We have applied this biosensor for high-throughput screening to discover novel upstre

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Mentoring Diversity for Inclusion and Equitye of calcium and lipids between both organelles, as well as in apoptosis and in autophagy signaling. Despite their importance, due to their dynamic and heterogeneous nature, we still lack understanding of the molecular composition, structure, and regulation of these structures. In this chapter, we i

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Freida Hopkins Outlaw,Janet Jacksonom a bioluminescent donor (luciferases) to a fluorescent acceptor. Since bioluminescence is an intrinsic phenomenon, BRET excludes the need for external illumination and serves as a powerful alternative to fluorescence-based systems. However, BRET has not been widely adopted for single-cell imaging

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