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Titlebook: Biological Electron Microscopy; Theory, Techniques, Michael J. Dykstra Book 19921st edition Michael J. Dykstra 1992 biological.chemistry.e

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https://doi.org/10.1007/978-3-8349-9651-0r significant hydrophilicity. For purposes of this chapter, however, we will limit our discussion almost exclusively to the epoxide sections, since these are the most commonly used resins for biological work. Any of the stains for these resins will work with much-reduced staining time on more water-miscible sections.
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https://doi.org/10.1007/978-3-663-02622-8-stopping capability (replicas produced by shadowing). This chapter will discuss these two added techniques for building subtractive contrast, pointing out the commonly used techniques and a few remedies to specific problems that may be encountered.
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Ultramicrotomy,ped). These requirements have resulted in the development of two basic instruments over the years: thermal- and mechanical-advance ultramicrotomes. An excellent and concise source of information on ultramicrotomy is the booklet “Ultramicrotomy: Frequent Faults and Problems,” by Sitte (1981). A more
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Support Films,otherwise fall through the openings in the grids. Support films are also used to cover grids with large open areas (e.g., 1 × 2 mm openings in slot grids), which can be used to view an entire 1-mm. section without obstruction from opaque grid bars. Finally, holey films can be prepared that are used
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