Titlebook: Biological Aging; Methods and Protocol Trygve O. Tollefsbol Book 2007 Humana Press 2007 DNA.Evolution.Microarray.Mutation.Proteomics.Telome

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,Andere Leistungen für Kinder (§ 65 EStG),us, the unicellular . is a simple model system that can provide significant insights into the human genetics and molecular biology of aging. Here, we describe the standard procedures to measure the chronological life span, including both the normal and calorie restriction paradigms.

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,Andere Leistungen für Kinder (§ 65 EStG), methods used to determine the replicative life span of single yeast “mother” cells and measure recombination frequency at the rDNA locus, and how to isolate and analyze the circular forms of DNA known as extrachromosomal rDNA circles (ERCs), which are a major cause of aging in . (.–.).

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https://doi.org/10.1007/978-3-8349-9205-5steps in our bud-scar-sorting system are the use of small microbeads, which allows successive rounds of purification and regrowth of the mother cells (M-cell), and utilization of flow cytometry to sort and isolate cells with a longer lifespan based on the number of bud scars specifically labeled with WGA.

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E.G. Haarman,G.A. van Essen,A.M. van Furthicroarray platforms, with radioactive and nonradioactive (fluorescent) labeling, are detailed in the manuscript. These in-depth protocols provide a background for the technical aspects of everyday work with DNA microarrays.

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1064-3745 lifespan using high-throughput screening systems.Biological Aging: Methods and Protocols investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protoco

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