Titlebook: Biolistic DNA Delivery in Plants; Methods and Protocol Sachin Rustgi,Hong Luo Book 2020 Springer Science+Business Media, LLC, part of Sprin

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https://doi.org/10.1007/978-3-531-90483-2y, versatility, and high efficiency. It has been a preferred method for investigating plant gene function in most monocot crops. The first transgenic sorghum plants were successfully regenerated through biolistic DNA delivery in 1993, with a relatively low transformation efficiency of 0.3%. Since th

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Toxoid-Vaccines

https://doi.org/10.1007/978-3-663-08069-5ted 12–16 days post-anthesis, the embryonic axis is removed, and the immature scutella are precultured for 1–2 days prior to particle bombardment. Gold particles are coated with plasmid DNA containing the gene(s) of interest plus a selectable marker gene, in this instance . (bialaphos resistance), a

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任命

https://doi.org/10.1007/978-3-531-90904-2ists, especially in the era of genome editing. The ability to deliver proteins or protein/RNA complexes into cells allows for producing gene-edited plants that are free of transgene integration in the genome. Here we describe a protocol for the delivery of a protein/gold particle mixture in plant ce

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