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Titlebook: Biofuels from Algae; Methods and Protocol Kristian Spilling Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 fl

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Measurement of Fluorescence for Monitoring Algal Growth and Health,rally positively correlated with chlorophyll . and can as such be used as a proxy for biomass. In addition, the proportion variable fluorescence of maximal fluorescence is a measure of photochemical efficiency, which is affected by stress in a very early stage and can as such be used as a proxy for
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Microalgae Lipid Staining with Fluorescent BODIPY Dye,ls and fluorescence measurements still should be clarified for the species that vary in cell wall complexity. Nevertheless, some general guidelines could be given, and a preliminary protocol of the method is provided based on the existing data. The semiquantification of lipid could be reliable if th
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Monitoring Microalgal Neutral Lipid Accumulation with Nile Red,imally, the fluorescent dye NR penetrates the microalgal cell wall staining the intracellular neutral lipids, and the measured fluorescence is linearly correlated to the neutral lipid concentration. Here I describe an optimization protocol for determining the optimal staining parameters for each new
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