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Titlebook: Bioconjugation Protocols; Strategies and Metho Christof M. Niemeyer Book 20041st edition Humana Press 2004

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期刊全称Bioconjugation Protocols
期刊简称Strategies and Metho
影响因子2023Christof M. Niemeyer
视频video
发行地址Includes supplementary material:
学科分类Methods in Molecular Biology
图书封面Titlebook: Bioconjugation Protocols; Strategies and Metho Christof M. Niemeyer Book 20041st edition Humana Press 2004
影响因子There are a number of outstanding volumes that provide a comprehensive overview of bioconjugation techniques. However, many of the conventional approaches to the synthesis of chemically modified protein conjugates lack efficient means to control the stoichiometry of conjugation, as well as the s- cific site of attachment of the conjugated moiety. Moreover, the recent dev- opments in microarray technologies as well as in nanobiotechnology—a novel field of research rapidly evolving at the crossroads of physics, chemistry, b- technology, and materials science—call for a summary of modern bioconjugation strategies to overcome the limitations of the classical approaches. Bioconjugation Protocols: Methods and Strategies is intended to provide an update of many of the classic techniques and also to introduce and summarize newer approaches that go beyond the pure biomedical applications of bioconjugation. The purpose of Bioconjugation Protocols: Methods and Str- egies is therefore to provide instruction and inspiration for all those scientists confronting the challenges of semisynthesizing functional biomolecular reagents for a wide variety of applications ranging from novel biomedical dia
Pindex Book 20041st edition
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Characterization of Endothelial Internalization and Targeting of Antibody—Enzyme Conjugates in Cell es and in vivo. Both specific delivery to cells and proper subcellular addressing of enzyme cargoes are important parameters of targeting. This chapter describes methodologies for evaluating the binding and internalization of labeled conjugates directed to endothelial surface adhesion molecules in c
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Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminaseloitation of the catalytic properties of the microbial transglutaminase from . species (MTGase), that is, acyl transfer between γ-carboxamide groups and various primary amines, new techniques for the enzymatic modification of proteins were developed. One example of bioconjugation is the biotinylatio
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Fluorescent Sample Labeling for DNA Microarray Analysesrom total ribonucleic acid (RNA) samples. Two procedures are based on first-strand complementary DNA synthesis by reverse transcription. Label is introduced either by direct incorporation of fluorescently labeled nucleotides or indirectly by incorporation of aminoally-dUTP and subsequent coupling of
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High-Density Labeling of DNA for Single Molecule Sequencingtarget into complementary flurophore-labeled DNA, and an exonuclease for the successive hydrolysis of the completely dye-labeled DNA. Recently, we found that the wild-type Klenow fragment of . DNA polymerase I is well-suited for the synthesis of DNA in a reaction set-up that contains exclusively spe
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