Titlebook: Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular; Eric Reid,J. D. Robinson,Ian D. Wilson Book 1988 Sp

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Analysis of Eicosanoids by GC-MS/MSlar substances often found in much higher concentration. Hence past assays of insufficient specificity have given erroneous values. GC-MS, which is more specific than RIA, is effective for structure elucidation and assay of eicosanoids and metabolites, yet may be insufficiently selective at low eico

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Quantitative Determination of Several Prostaglandins and Thromboxane B2 in the Isolated Perfused Rated rat brain, perfused with a synthetic fluoro-carbon perfusion medium. After perfusion, the brains were frozen in isopentane at −56°, freeze-dried at −40° and pulverized (liq. N.). The powder (~100 mg) was spiked with 4 deuterated analytes and extracted with methanol/water. The supernatants were ap

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Analytical Methods for [3H]-Enisoprost, an Anti-Secretory PGE1 Analogue, and its MetabolitesLRC* were used. These methods were scaled up to allow isolation and purification of urinary metabolites. These were identified by GC-MS of their ME-MO-TMS and PFB-MO-TMS derivatives. The latter provided mol. wt. data from the intense [M-PFB]. ions formed by NCI with ammonia, and the former provided

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Identification of a New Metabolite of Leukotriene B4 by RP-HPLC and GC-MS Analysisunds precludes separating all of them within one system. Therefore RP-HPLC systems have been developed to isolate particular groups of compounds such as peptidoLT’s or LTB. and its isomers. A RP-HPLC system now described allows the separation of a new metabolite of LTB. from LTB. and its trans isome

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Radioimmunoassay of Eicosanoids: Its Application in the Development of Anti-Inflammatory and Analgesy including regulation of microvascular blood flow and permeability, leukocyte chemotaxis, hyperalgesia and anaphylaxis. Consequently, in recent years considerable effort has been expended in the search for agents that block the synthesis and antagonize the actions of these autocoids. Most of the bi

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The Stereochemical Analysis of Monohydroxyfatty Acids, Particularly 12-Hydroxy-5,8,10,14-Eicosatetrahanism of formation of these compounds stimulated investigation of their stereochemistry. Chiral methods using HPLC have been developed for separation and quantification of the hydroxyl enantiomers as well as for their multi-mg preparation.

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Analysis of the Actions of Anti-Inflammatory Drugs, with Particular Reference to Effects on Interleustimulated or unstimulated leucocytes. Assays developed for two main IL’s, viz. IL-1 and IL-2, include cell responses such as production of other mediators, or reactions (e.g. proliferation, aggregation) whose specificity depends on the responsiveness of the cell type as well as the purity of the ex

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Immunoassay of Beclomethasone 17,21-Dipropionate and Metabolites levels down to 0.1 (plasma) or 1 (urine) ng/ml. The 3-O-carboxymethyloxime derivative of BDP was coupled by a mixed-anhydride reaction to bovine serum albumin (BSA) for raising a rabbit antiserum, or to [..] iodohistamine to provide a radiolabel. Known BDP metabolites and endogenous steroids had ne