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Titlebook: Bifidobacteria; Methods and Protocol Douwe van Sinderen,Marco Ventura Book 2021 Springer Science+Business Media, LLC, part of Springer Natu

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期刊全称Bifidobacteria
期刊简称Methods and Protocol
影响因子2023Douwe van Sinderen,Marco Ventura
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Bifidobacteria; Methods and Protocol Douwe van Sinderen,Marco Ventura Book 2021 Springer Science+Business Media, LLC, part of Springer Natu
影响因子.This volume provides current protocols that can be used in various experimental settings involving bifidobacteria. Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial isolates, sequencing and annotate genomes, physiologically characterize bifidobacteria, and methods on the genetic manipulation of bifidobacterial strains.  Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. ..Authoritative and cutting-edge,  .Bifidobacteria: Methods and Protocols. .aims to be a useful practical guide to researches to help further their study in this field. .
Pindex Book 2021
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书目名称Bifidobacteria影响因子(影响力)




书目名称Bifidobacteria影响因子(影响力)学科排名




书目名称Bifidobacteria网络公开度




书目名称Bifidobacteria网络公开度学科排名




书目名称Bifidobacteria被引频次




书目名称Bifidobacteria被引频次学科排名




书目名称Bifidobacteria年度引用




书目名称Bifidobacteria年度引用学科排名




书目名称Bifidobacteria读者反馈




书目名称Bifidobacteria读者反馈学科排名




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Transformation,ing selective properties onto strains as well as allowing the user to introduce or knock out/in selected genes for phenotypic characterization purposes. We have generalized on the plasmid chosen for transformation and antibiotic selection marker, but the protocol is versatile in this respect and we
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Site-Directed Mutagenesis of , Strains,jority of bifidobacterial strains represents a significant roadblock for the study of gene function and expression in these potential probiotics. Genetic tools for generating mutants are difficult to develop for bifidobacteria, as they require workarounds for obstacles such as low transformation eff
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Determination of Bifidobacterial Carbohydrate Utilization Abilities and Associated Metabolic End Prd polysaccharides. Different bifidobacterial strains may possess different carbohydrate utilization abilities. These metabolic abilities can be studied using classical bacterial growth assessment methods, such as measurement of changes in optical density or acidity of the culture in the presence of
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