Titlebook: Behavioral Neurogenetics; Daisuke Yamamoto Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer

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Combined in Situ Hybridization Chain Reaction and Immunostaining to Visualize Gene Expression in Whd protocol that sequentially combines in situ hybridization chain reaction (HCR) and immunostaining to detect mRNA and protein expression in whole-mount . larval and adult central nervous systems. We demonstrate the application of in situ HCR in comparisons of nervous system gene expression between

ULCER

Mapping Brain-Wide Mesoscale Connectome from Single Animals with BRICseq,rcuit function and animal behaviors. Therefore, understanding the logic that relates distinct modalities of the nervous system, including connectome, neural activity, gene expression, and animal behaviors, is one of the core questions in neuroscience. Here I describe methods of BRICseq, which allows

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Measurement of Thermoregulatory Behavior in ,the fruit fly (.) has markedly contributed to the study of behavioral genetics. Behavioral analyses using . have revealed the sensory neurons, sensor molecules, and other factors including energy metabolism that are closely linked to thermoregulation. In this chapter, we describe the equipment and p

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A Method for Selective Breeding to Domesticate Mice,lecting animals that exhibit an appropriate phenotype in the process of breeding in human history. Several experimental approaches have been applied to domesticated animals from wild stock in several species. Tameness, a major factor in domestication, is composed of two components—active and passive

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