Titlebook: Basophils and Mast Cells; Methods and Protocol Bernhard F. Gibbs,Franco H. Falcone Book 2014 Springer Science+Business Media New York 2014

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Purification of Basophils from Peripheral Human Bloodin 1879. From the first published attempts in the late 1960s, it took half a century to develop robust protocols able to provide sufficient numbers of pure, functionally unimpaired basophils. The existing protocols for basophil purification exploit those properties of basophils which distinguish the

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Mast Cell Purification Protocolsd from different sources. Methods for isolating mast cells from a variety of tissues have been in existence for a number of years although, over time, these methodologies have been refined. We have had considerable experience studying mast cells isolated from human lung tissue. It is for this reason

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Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cellsral blood can provide information on immune mechanisms of known, selected individuals. With the method presented here eight million mast cells can be cultured from ca. one million stem cells purified from one unit (450 mL) of human peripheral blood. Culture with IgE and IL4 optimizes an allergic phe

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Mast Cell and Basophil Cell Lines: A Compendiumnesis of allergy and inflammation, our understanding of mast cell and basophil biology is still relatively scarce. The practical difficulty in obtaining a sufficient number of purified primary cells from biological samples has slowed down the process of reaching a full understanding of the physiolog

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Detection of Mast Cells and Basophils by Immunohistochemistryntification of these cell types using such methods. These techniques have now been largely supplanted by immunohistochemical procedures with monoclonal antibodies directed against unique constituents of these cell types. Immunohistochemistry with antibodies specific for the granule protease tryptase

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Measuring Histamine and Cytokine Release from Basophils and Mast Cellscribe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detecti