Titlebook: Basic and Clinical Applications of Flow Cytometry; Proceeding of the 24 Frederick A. Valeriote,Alexander Nakeff,Manuel Val Conference proce

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Stephan Eggersglüß,Rolf Drechslereoplastic populations. In recent years, it has become clear that cell growth and proliferation are controlled by an elaborate homeostatic mechanism which is balanced by numerous intracellular and extracellular signals. It is hardly surprising then that oncogenes and tumor suppressor genes which medi

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Stephan Eggersglüß,Rolf Drechslerment of flow cytometry, a look back through the literature reveals that the field has not developed precisely as its pioneers envisioned. While flow cytometry is now widely used in oncology, reexamining some longstanding unsolved problems and neglected areas from the vantage point of the present may

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The Lithic Assemblage of Level Jconclusions reached by these studies. Techniques used to produce a monodisperse suspension of cells or nuclei from a tumor are a principal source of this variability. Dissociation of either fresh or fixed, paraffin-embedded tissue requires disruption of cell-cell and cell-matrix attachments. Dissoci

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The Lithic Assemblage of Level Jse despite conventional therapy (1–4). SJL lymphomas provide a model for development of a new treatment for this disease by revealing the existence of a novel target for therapy, nonmalignant lymphocytes upon which the tumor cells are dependent for growth (5).

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Josep Vallverdú-Poch,Marie-Agnès Courtyhromosomes are stained with two DNA-specific fluorochromes, Hoechst 332 58 which has specificity for AT-rich DNA and Chromomycin A3 with specificity for GC-rich DNA. In the flow cytometer, the chromosomes are passed sequentially through two spatially separated laser beams, the first operated using t