Titlebook: Basic Techniques in Molecular Biology; Stefan Surzycki Book 2000 Springer-Verlag Berlin Heidelberg 2000 DNA.DNA Isolierung.DNA sequencing.

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DNA Cloning. General Consideration,ification of DNA fragments that can be used in subsequent experiments. Cloning involves construction of hybrid DNA molecules that are able to self-replicate in a host cell, usually bacteria. This is accomplished by inserting DNA fragments into a plasmid or bacteriophage cloning vector, introducing t

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DNA Sequencing, generates data that are not biased by previous assumption, hypotheses or experimental design. It is therefore not surprising that DNA sequencing has revealed many unexpected facts concerning gene structure, regulation of gene expression, organization of genomes, as well as, discovered new genes nev

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https://doi.org/10.1007/978-1-4615-5611-4ridized with a specific, labeled probe and results are visualized by autoradiography. The method is sensitive enough to detect mRNA present in the cell in 5 to 10 copies when analyzing 10 μg of total RNA. As little as one copy of mRNA per cell can be detected when 1 to 2 μg of poly-A. mRNA is xanalyzed(Davis et all, 1994).

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Abhinav Sarje,Xiaoye S. Li,Alexander Hexemering whole genomes and their function (Hieter and Boguski, 1997). Knowing the complete sequences of a dozen microbial genomes and expected completion of sequencing of many other organisms, including humans, will without doubt change how biology is done in the future (Doolittle, 1998; DeRisi et al. 1997; Tatusow et al., 1997).

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