Titlebook: Basic Protein and Peptide Protocols; John M. Walker Book 1994 Humana Press 1994

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The Lowry Method for Protein Quantitation,itive to the amino acid composition of the protein, and absolute concentrations cannot be obtained. The procedure of Lowry et al. (.) is no exception, but its sensitivity is moderately constant from protein to protein, and it has been so widely used that Lowry protein estimations are a completely ac

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The Bicinchoninic Acid (BCA) Assay for Protein Quantitation,to Cu. under alkaline conditions (..). The Cu. is then detected by reaction with BCA. The two assays are of similar sensitivity, but since BCA is stable under alkali conditions, this assay has the advantage that it can be carried out as a one-step process compared to the two steps needed in the Lowr

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The Bradford Method for Protein Quantitation, Bradford (.) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of b

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