期刊全称 | Barley | 期刊简称 | Methods and Protocol | 影响因子2023 | Wendy A. Harwood | 视频video | | 发行地址 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key notes and expert implementation advice | 学科分类 | Methods in Molecular Biology | 图书封面 |  | 影响因子 | This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and easy-to-use, .Barley: Methods and Protocols. serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.. | Pindex | Book 2019 |
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