Titlebook: Bacteriophages; Methods and Protocol Martha R.J. Clokie,Andrew M. Kropinski,Rob Lavigne Book 2018 Springer Science+Business Media LLC 2018

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Geschäftsprozesse optimal gestalten efficiently purified and concentrated..This protocol can be used to determine the optimal buffers, stationary phase chemistry and elution conditions, as well as the maximal capacity and recovery of the columns.

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Purification of Bacteriophages Using Anion-Exchange Chromatography efficiently purified and concentrated..This protocol can be used to determine the optimal buffers, stationary phase chemistry and elution conditions, as well as the maximal capacity and recovery of the columns.

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Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes, achieving this standard still requires a significant amount of human oversight and expertise. This chapter describes our workflow for sequencing, assembling, and finishing phage genomes to a high standard by the NGS platforms Illumina, Ion Torrent, and 454.

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Analyzing Genome Termini of Bacteriophage Through High-Throughput Sequencing bacteriophage genome termini, which is based on the large data of HTS. With this method, the termini of the bacteriophage genome can be efficiently and reliably identified as a by-product of bacteriophage genome sequencing, by solely analyzing the sequence statistics of the raw sequencing data (reads), without any further lab experiments.

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Annotation of Bacteriophage Genome Sequences Using DNA Master: An Overviewiction can be largely accomplished through the use of several freely available programs. However, manual inspection and refinement is essential to the production of the most accurate genome annotations. Here, we describe an overview of the annotation of a bacteriophage genome sequence using the freely available program DNA Master.

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