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Titlebook: Bacteriophages; John Douglas Book 1975 John Douglas 1975 behavior.biology.development.molecular biology.nucleic acids

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https://doi.org/10.1007/978-1-4899-3418-5behavior; biology; development; molecular biology; nucleic acids
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The latent period and the eclipse,en it bursts, yet, if the cell is broken open, no phage can be extracted from it. What becomes of the phage during this ‘eclipse phase’? The answer to this question has been obtained from three main lines of investigation. Again, the T-even phages predominate.
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Sonia Lippke,Elise-Marie Dilgermaking it important to define certain terms at the outset. Survival may imply survival as an object recognisable in the electron microscope as a phage, i.e. not utterly destroyed, but most workers take it to mean the retention of infectivity as shown by the formation of plaques or the clearing of broth cultures.
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Lysis,yme, that attacks the murein of the cell wall, weakening it so that it bursts and liberates the phage within. In some cases the dissolution of the wall is very nearly complete, in others a substantial amount of debris remains. Lysis can be detected in several ways.
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