Titlebook: Bacteriophages; Methods and Protocol Martha R. J. Clokie,Andrew Kropinski,Rob Lavigne Book 2019 Springer Science+Business Media, LLC, part

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Jürgen Baumert,Hans-Peter Füsselof revealing the metabolic state of mycobacterial cells and therefore their response to antibiotics. Here we described a simple and rapid method for drug susceptibility testing (DST) of . using a fluorescence microscope, a flow cytometer, or a fluorimeter in a convenient multiwell format.

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Jürgen Baumert,Hans-Peter Füssel a narrow subset of diagnostic settings. More recently, however, advances in DNA sequencing and the introduction of more sensitive reporter systems have enabled novel engineering methods, which in turn have broadened the scope of modern phage diagnostics. Here we describe advanced methods to enginee

mucous-membrane

Max-Emanuel Geis,Daniel Krausnickoving medical treatments. Here we describe its use for introduction of phage genomic DNA into . cells, including preparation of electrocompetent cells, electric pulse optimization and recovery of electrotransformed cells. The technique can also be adapted for other bacterial species.

aneurysm

Max-Emanuel Geis,Daniel Krausnickrgeted gene is cloned in a . shuttle vector. Using an in vitro enzymatic procedure dependent on mutant oligonucleotide primers, a mutation is inserted into the cloned gene, replacing an early lysine codon (AAA or AAG) with a nonsense codon (TAG or TAA). The mutant plasmid is recovered by transformat

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Max-Emanuel Geis,Daniel Krausnicklation method. Here, we demonstrate that T4-like phages can be efficiently isolated by affinity chromatography. This approach employs specific affinity tags (GST (glutathione S-transferase) or His-tag) that allow for the isolation of the phage. These affinity tags are exposed on the phage head using

Irrepressible

Jürgen Baumert,Hans-Peter Füssel of bacterial pathogens. Whole phage can carry reporter genes to alter the phenotype of the target pathogen. Phage can also act as staining agents or the progeny of the infection process can be detected. Alternatively, using phage components as probes offer advantages over whole phage particles, inc

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https://doi.org/10.1007/978-3-642-15523-9sualization of enzymatically active protein species separated by molecular mass. The strategy is to perform SDS-PAGE on the proteins in question while including an opaque substrate of the enzyme embedded within the polyacrylamide gel. Here, we describe a zymogram protocol for phage lytic proteins (p

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