Titlebook: Bacteriophage Therapy; From Lab to Clinical Joana Azeredo,Sanna Sillankorva Book 2024Latest edition The Editor(s) (if applicable) and The A

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https://doi.org/10.1007/978-3-658-14686-3iophage therapy is considered an additional approach to the treatment of infected wounds. Here, we describe the basic method of application of bacteriophages for the treatment of infected diabetic foot ulcers, including very large ones.

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Isolation of Bacteriophages for Clinically Relevant Bacteriaprocedures, and following enrichment, it is common to obtain more than a single phage in a sample. This chapter describes a simple method for the enrichment and isolation of bacteriophages from liquid and solid samples that can be adapted for different clinically important aerobic bacteria.

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Bacteriophage Control of Infectious Biofilmscontrol biofilm-related infections. This chapter describes a series of standard procedures that can be used to study the potential of bacteriophages for biofilm control, from biofilm formation to bacteriophage treatment and evaluation of its efficacy.

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Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-Specific Antibodiesols applicable in studies of the ability of bacteriophage to induce specific antibodies; immunization to whole virions or to isolated phage proteins has been included. The protocols have been divided into three parts: purification, immunization, and detection (enzyme-linked immunosorbent assay, ELISA).

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Methods in Molecular Biologyhttp://image.papertrans.cn/b/image/180345.jpg

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Bacteriophage Therapy978-1-0716-3523-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Hans J. Gießmann,Bernhard Rinkether time-efficient, not labor-intensive, and cost-effective methods have been developed..This chapter describes the methodology used to assess the susceptibility of planktonic cultures of bacteria to phage infection and to study their interactions over time by flow cytometry.