Titlebook: Bacterial Virulence; Methods and Protocol Ohad Gal-Mor Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license t

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Olaf Dörner M.A.,Burkhard Schäffer that mimics the histopathology observed in humans, namely the destruction of soft tissue, a paucity of infiltrating neutrophils, and the presence of many gram-positive cocci at the center of the infection.

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Generation of Markerless Gene Deletion Mutants in , Using a Mutated , for Counterselectioncient allelic exchange gene deletion method in the bacterial pathogen .. Combining vector generation by Gibson assembly with a counterselection system based on the mutated phenylalanine synthetase (.*) makes the generation of gene deletion mutants straightforward and time efficient.

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https://doi.org/10.1007/978-1-0716-1971-1Bacterial pathogenesis; Pathogens; Infectious diseases; Antibiotic resistant bacteria; Drug resistance; M

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Karin Fischer,Gerhard Hauck,Manuela Boatcăcient allelic exchange gene deletion method in the bacterial pathogen .. Combining vector generation by Gibson assembly with a counterselection system based on the mutated phenylalanine synthetase (.*) makes the generation of gene deletion mutants straightforward and time efficient.

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Ohad Gal-MorIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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