| 期刊全称 | Bacterial Sensors | | 期刊简称 | Synthetic Design and | | 影响因子2023 | Jan Roelof Meer | | 视频video | | | 学科分类 | Synthesis Lectures on Synthetic Biology | | 图书封面 |  | | 影响因子 | Bacterial reporters are live, genetically engineered cells with promising application in bioanalytics. They contain genetic circuitry to produce a cellular sensing element, which detects the target compound and relays the detection to specific synthesis of so-called reporter proteins (the presence or activity of which is easy to quantify). Bioassays with bacterial reporters are a useful complement to chemical analytics because they measure biological responses rather than total chemical concentrations. Simple bacterial reporter assays may also replace more costly chemical methods as a first line sample analysis technique. Recent promising developments integrate bacterial reporter cells with microsystems to produce bacterial biosensors.This lecture presents an in-depth treatment of the synthetic biological design principles of bacterial reporters, the engineering of which started as simple recombinant DNA puzzles, but has now become a more rational approach of choosing and combining sensing, controlling and reporting DNA ‘parts‘. Several examples of existing bacterial reporter designs and their genetic circuitry will be illustrated. Besides the design principles, the lecture also fo | | Pindex | Book 2011 |
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发表于 2025-3-21 17:06:53
