Titlebook: Bacterial Regulatory RNA; Methods and Protocol Véronique Arluison,Claudio Valverde Book 2024Latest edition The Editor(s) (if applicable) an

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Investigation of sRNA-mRNA Interactions in , In Vivoher established or significantly improved in our group and successfully employed to characterize a number of sRNA/target mRNA systems in .. Whereas in Chap. 8, we describe a combination of in vitro methods, e.g., EMSA and RNA secondary structure probing, we focus here on the investigation of RNA-RNA

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In Vitro Methods for the Investigation of sRNA-mRNA Interactions in 1, 2). Here, we describe in vitro methods for the analysis of sRNA/mRNA interactions. All these methods have been either elaborated or significantly improved in our group and successfully applied to characterize a number of sRNA/target mRNA systems in . for which we provide examples from our own wo

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Evaluation of 5′-End Phosphorylation for Small RNA Stability and Target Regulation In Vivoary transcripts, undergo dephosphorylation or originate via processing. Often, 5′ groups hallmark RNAs for rapid decay, but whether this also applies to sRNAs is little explored. Moreover, the sRNA 5′P group could activate endoribonuclease RNase E to cleave the base-paired target RNA, but a tool for

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Directed Screening for sRNA Targets in , Using a Plasmid Libraryility. This posttranscriptional regulation has been shown to be essential and critical for bacterial physiology. One of the challenges of studying sRNA signaling is identifying the sRNA regulators of specific genes. Here, we describe a protocol for making an sRNA expression library and using this li