Titlebook: Bacterial Polysaccharides; Methods and Protocol Inka Brockhausen Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20

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Direct Conjugation of Bacterial Polysaccharides to Proteins by Squaric Acid Chemistry,s a two-step process consisting of labeling the polysaccharide with a squarate group and a reaction of the squarate formed with protein. The intermediate squarate derivative and the product glycoconjugate can be easily purified using centrifugal filtration devices. This method is experimentally simp

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Chemical Modification of the N-Acetyl Moieties of Hyaluronic Acid from , for Studies in Cytokine Prian systems. Here, we describe the treatment of bacterial hyaluronic acid by hydrazine or NaOH to yield smaller partially deacetylated polymers. These N-deacetylated polymers can be reacylated with acyl anhydrides to yield substituted hyaluronic acid derivatives of equivalent size and equimolar N-ac

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Designing Glycosyltransferase Expression Constructs for Improved Purification, Protein Yield, and Cvariety of other domains, including those that help recruit them to the membrane. Structural and functional characterization of these proteins is often simplified by making a subconstruct that is better behaved in solution, and perhaps monofunctional. In this chapter we review bioinformatics tools a

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Enzymatic Synthesis of Repeating Unit Oligosaccharides of , O104,arides of ECO104 consist of repeating units with the structure [4Galα1-4Neu5,7,9Ac3α2-3Galβ1-3GalNAcβ1-].. These repeating units are assembled sequentially by specific glycosyltransferases on a diphosphate-undecaprenol intermediate. Internal structures include mimics of the human T and sialyl-T anti

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Sulfhydryl Labeling as a Tool to Investigate the Topology of Membrane Proteins Involved in Lipopolygions, delimit the protein orientation in the membrane, the number of transmembrane segments, and the position of critical amino acids (whether exposed to the solvent or embedded in the lipid bilayer). Elucidating the topology of bacterial integral membrane proteins typically involves the constructi

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