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Titlebook: Bacterial Persistence; Methods and Protocol Natalie Verstraeten,Jan Michiels Book 2021Latest edition The Editor(s) (if applicable) and The

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发表于 2025-3-21 19:45:39 | 显示全部楼层 |阅读模式
期刊全称Bacterial Persistence
期刊简称Methods and Protocol
影响因子2023Natalie Verstraeten,Jan Michiels
视频video
发行地址Includes cutting-edge methods and protocol.Provides step-by-step detail essential for reproducible result
学科分类Methods in Molecular Biology
图书封面Titlebook: Bacterial Persistence; Methods and Protocol Natalie Verstraeten,Jan Michiels Book 2021Latest edition The Editor(s) (if applicable) and The
影响因子.This volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial. .cultures, strategies to enrich and resuscitate persister subpopulations, single-cell approaches for visualizing and characterizing persisters, omics techniques and cellular and animal models for studying persistence. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. ..Authoritative and cutting-edge, .Bacterial Persistence: Methods and Protocols, Second Edition .aims to be a useful practical guide to researchers to help further their study in this field..
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https://doi.org/10.1007/978-1-0716-1621-5Microfluidics; flow cytometry; Metabolomics; aminoglycosides; population dynamics
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https://doi.org/10.1007/978-3-531-92015-3alled persister phenotype, which makes it difficult to study this health-threatening phenotype. We recently found that certain abrupt nutrient shifts generate . populations that consist almost entirely of antibiotic-tolerant cells. These nearly homogeneous persister cell populations enable assessmen
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Rudolf Tippelt,Bernhard Schmidtlly, the abundance of persister cells is very low (about 1 in 10. cells) in actively growing bacterial populations. Therefore, in order to study bacterial persistence mechanisms for therapeutics development, persister cells need to be enriched from a larger culture. Here, we describe three enrichmen
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Henning Pätzold,Albert Schmelzerr the antibiotic treatment. Therefore, time-lapse microscopy is the gold-standard method to investigate this phenomenon. Here, we describe an exhaustive procedure for acquiring single-cell data which is particularly suitable for persister cell analysis but could be applied to any other fields of res
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