Titlebook: Bacterial Pathogenesis; Methods and Protocol Pontus Nordenfelt,Mattias Collin Book 2017 Springer Science+Business Media New York 2017 strep

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虚弱的神经

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Detection of Intracellular Proteins by High-Resolution Immunofluorescence Microscopy in ,osed antigens can easily be determined using fluorescent antibodies, localization of intracellular antigens requires permeabilization of the bacterial cell wall and membrane. Here, we describe an immunofluorescence protocol tailored specifically for ., applying the phage lysin PlyC for cell wall per

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Bacterial Pathogenesis978-1-4939-6673-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Estrogen

Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomicsproteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host–pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest for further studies.

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breadth

Pontus Nordenfelt,Mattias CollinIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts.Includes supplementary material