Titlebook: Bacterial Pathogenesis; Methods and Protocol Pontus Nordenfelt,Mattias Collin Book 2023Latest edition The Editor(s) (if applicable) and The

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Elucidating the Stoichiometries of Host–Pathogen Protein Interactions with Mass Photometryas exemplar proteins. The methodology described herein confirmed a 1:1 binding stoichiometry for the M53–plasminogen complex. Additionally, MP was used to identify the oligomerization state, homogeneity, purity, and approximate molecular weights of each protein.

Bernstein-test

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https://doi.org/10.1007/978-3-322-84764-5ro assay. To reduce potentially toxic effect on the host cell, the growth and production phases were separated by using the inducible promoter . to control recombinant gene expression. The protocol provided may be adopted for other bacterial proteases through minor modifications of the fused protein.

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Expression of the Bacterial Enzyme IdeS Using a GFP Fusion in the Yeast ro assay. To reduce potentially toxic effect on the host cell, the growth and production phases were separated by using the inducible promoter . to control recombinant gene expression. The protocol provided may be adopted for other bacterial proteases through minor modifications of the fused protein.

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Pontus Nordenfelt,Mattias CollinIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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