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Titlebook: Bacterial Growth and Lysis; Metabolism and Struc M. A. Pedro,J.-V. Höltje,W. Löffelhardt Book 1993 Springer Science+Business Media New York

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The Muropeptide Composition of the Peptidoglycan of , Determined with Reversed-Phase High Performanc965), the high resolution technique of reversed-phase high performance liquid chromatography (HPLC), recently developed by Glauner et al. (1988), has not been applied to staphylococcal cell walls until now. In this communication, we report the results of studies in which the HPLC method, adapted to
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Distribution of ,-Acetylation in the Peptidoglycan from , 19owed by the release (saponification) and quantitation of acetate. Since this methodology is laborious and in addition, the ester-linked acetyl moiety is highly labile, it is not surprising that the prevalence of .-acetylated peptidoglycan among the eubacteria is largely unknown. The only microorgani
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Crystalline Bacterial Cell Surface Layers and their Application Potentialscal group of walled eubacteria and archaebacteria (Fig. 1) by electron microscopy of either freeze-etched, freeze-dried and shadowed, negatively stained, or thin sectioned preparations (for reviews see Sleytr, 1978; Sleytr and Messner, 1983, 1988 .; Sleytr ., 1988; Messner and Sleytr, 1992).
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Identification of the , and , Genes Coding for Cytoplasmic Peptidoglycan Synthetases in the 90-Min Racetylglucosamine (UDP-GlcNAc), UDP-GlcNAc-enolpyruvate, UDP-.-acetylmuramic acid (UDP-MurNAc) and its four peptide derivatives from UDP-MurNAc-L-Ala to UDP-MurNAc-L-Ala-ǒ-D-Glu-.-DAP-D-Ala-D-Ala. Their sequential formation is catalyzed by a set of eight highly specific enzymes which were earlier st
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Peptidoglycan Synthesis in ,lic acid (DAP) into peptidoglycan to incorporation of radioactive amino acids into protein (Cooper, 1988). The observed ratio of DAP incorporation to amino acid incorporation during the division cycle led to the development of a consistent description of peptidoglycan synthesis. It was proposed that
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Biosynthesis of Peptidoglycan in ,: Regulation of Transpeptidation by Acceptor Peptidetidases. In contrast to cross-linking enzymes from other bacteria, the transpeptidase from this gram-positive organism is essentially insensitive to inhibition by penicillin (Hammes, 1976). Thus, the target of penicillin in . is different from that identified by Tipper and Strominger (1965) for . an
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