Titlebook: Bacterial Chromatin; Methods and Protocol Remus T. Dame Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 nucleo

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娴熟

https://doi.org/10.1007/978-1-4615-5295-6mple and effective method named .nome .ootprinting with high-throughput .uencing (GeF-seq) to determine binding sites of DBPs at single base-pair resolution. GeF-seq detects binding sites of DBPs as sharp peaks and thus makes it possible to identify the recognition sequence in each “binding peak” more easily and accurately than using ChIP-seq.

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The Historian’s Craft in the “Periphery”s makes it possible to measure binding properties. We use the bacterial protein Integration Host Factor (IHF) as an example to show how specific binding to DNA can be measured. Moreover, we show a new intuitive quantitative approach to displaying data obtained via TPM.

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Observing Bacterial Chromatin Protein-DNA Interactions by Combining DNA Flow-Stretching with Single-w the binding of bacterial chromatin proteins can be correlated with DNA condensation. Lastly, we describe the DNA motion capture assay, which allows one to probe the mechanism of DNA condensation by tracking how different segments of a flow stretched DNA are compacted by bacterial chromatin proteins.

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Imaging of Transcription and Replication in the Bacterial Chromosome with Multicolor Three-Dimensionteins, (2) imaging the transcription and replication machineries at single-cell levels, (3) performing imaging experiments to capture the spatial organization of the transcription machinery and the nucleoid, and (4) image acquisition and analysis.