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Titlebook: Bacterial Artificial Chromosomes; Volume 1: Library Co Shaying Zhao,Marvin Stodolsky Book 2004 Humana Press 2004 BAC.Chromosom.DNA.DNA sequ

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期刊全称Bacterial Artificial Chromosomes
期刊简称Volume 1: Library Co
影响因子2023Shaying Zhao,Marvin Stodolsky
视频video
发行地址Includes supplementary material:
学科分类Methods in Molecular Biology
图书封面Titlebook: Bacterial Artificial Chromosomes; Volume 1: Library Co Shaying Zhao,Marvin Stodolsky Book 2004 Humana Press 2004 BAC.Chromosom.DNA.DNA sequ
影响因子For both volumes:..Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.
Pindex Book 2004
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Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging,ery low frequency. On the other hand, in vitro packaging limits the size of the DNA based on the phage head stability. However, in vitro package is the most efficient method for introducing large-insert clones.
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BAC Finishing Strategies,PCI-13 from P. de Jong (.) were fingerprinted, and the resulting maps of these overlapping clones have been integrated with clone maps of individual chromosomes generated by other centers (.). Sets of minimal overlapping clones have been selected for sequencing from the resulting maps.
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George J. Vachtsevanos,Kimon P. Valavanisse it relies on characterization of random clones in libraries comprising YACs or BACs. Even if a YAC or BAC contains an entire gene, the specific recovery of the gene was an arduous process. Often a gene is available as a contiguous set of fragments that must be pieced together.
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Sexual Minority Groups and Urban Healthe found that templates produced through RCA yield more consistent and higher-quality sequence than identical templates generated from plasmid-prep methods. The protocol is simple, amenable to high throughput, and currently in use at the DOE Joint Genome Institute (Walnut Creek, CA) for the daily production of 30,000 sequencing templates.
发表于 2025-3-22 22:50:54 | 显示全部楼层
Tree Roots versus Sidewalks and Sewers,e the sequences contained in them. Thus, both physical and functional mapping objectives would be facilitated by an efficient strategy for making nested deletions from one end of the insert DNA in a BAC or PAC clone.
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Rolling Circle Amplification for Sequencing Templates,e found that templates produced through RCA yield more consistent and higher-quality sequence than identical templates generated from plasmid-prep methods. The protocol is simple, amenable to high throughput, and currently in use at the DOE Joint Genome Institute (Walnut Creek, CA) for the daily production of 30,000 sequencing templates.
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