Titlebook: BCL-2 Family Proteins; Methods and Protocol Evripidis Gavathiotis Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2

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书目名称BCL-2 Family Proteins读者反馈




书目名称BCL-2 Family Proteins读者反馈学科排名

Mucosa

Liposomal Permeabilization Assay to Study the Functional Interactions of the BCL-2 Family, be quantified by the increase in fluorescence intensity as the fluorophore and quencher dissociate. The liposomal permeabilization assay has been used to delineate interactions among BCL-2 family members as well as to characterize peptides, small molecules, and lipids that modulate the function of

确保

Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs, a dominant inhibitor of programmed cell death and a major anticancer drug target. The protocols are relatively straightforward. Provided care is taken to ensure protein integrity and sample homogeneity, BCL-XL can be readily reconstituted in nanodiscs, with its hydrophobic C-terminal tail anchored

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1064-3745 essary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .BCL-2 978-1-4939-8861-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Heather Winskel,Theeraporn Ratitamkul be quantified by the increase in fluorescence intensity as the fluorophore and quencher dissociate. The liposomal permeabilization assay has been used to delineate interactions among BCL-2 family members as well as to characterize peptides, small molecules, and lipids that modulate the function of

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Liquid Metal Biomedical Imaging,ds. Finally, we describe how to generate MOMP-resistant cell lines, using CRISPR-Cas9 mediated deletion of BAX and BAK. Facilitating the investigation of mitochondrial apoptosis, mito-priming provides a clean, robust way to induce mitochondrial apoptosis both in vitro and in vivo.

rheumatism

Application of Mito-Priming to Generate BCL-2 Addicted Cells,ds. Finally, we describe how to generate MOMP-resistant cell lines, using CRISPR-Cas9 mediated deletion of BAX and BAK. Facilitating the investigation of mitochondrial apoptosis, mito-priming provides a clean, robust way to induce mitochondrial apoptosis both in vitro and in vivo.