Titlebook: B Cell Receptor Signaling; Methods and Protocol Chaohong Liu Book 2018 Springer Science+Business Media, LLC 2018 Drosophila.S2 Schneider ce

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Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors,B cell receptor signaling leads to B cell activation and differentiation. Recombinant retrovirus-based transduction is the most efficient method to deliver exogenous genes for expression in B cells. In this chapter, we describe streamlined protocols for using recombinant retroviral vectors to transduce both murine primary B cells and B cell lines.

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In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Noded one of these experimental models, which uses highly fluorescent particulate antigen and B cell receptor (BCR)-transgenic B cells labeled with long-term fluorescent probes, combined with confocal and multiphoton microscopy.

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Study B Cell Antigen Receptor Nano-Scale Organization by In Situ Fab Proximity Ligation Assay,assay (Fab-PLA), we now obtain proofs for the dissociation activation of BCRs and start to gain insight into how the relocalization of B cell surface signaling molecules could activate the cells. This chapter describes our methods to study the nano-scale organization of B cell surface receptors and co-receptors with Fab-PLA.