Titlebook: Axon Growth and Regeneration; Methods and Protocol Andrew J. Murray Book 2014 Springer Science+Business Media New York 2014 Axon growth.Axo

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Analysis of Calcium Signals in Steering Neuronal Growth Cones In Vitroion often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.

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In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regenerationos. Here we report a rapid and efficient approach to transfect adult mouse dorsal root ganglion neurons in vivo with precise spatiotemporal control via electroporation. This approach will allow both gain- and loss-of-function experiments in vivo to study the function of adult sensory neurons, such as sensory axon regeneration.

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Axon Growth and Regeneration978-1-4939-0777-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

尖叫

Milan Gopal,Tariq Abbas,J. L. Pippi Salleion often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.

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1064-3745 ctions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Practical and r978-1-4939-4584-9978-1-4939-0777-9Series ISSN 1064-3745 Series E-ISSN 1940-6029