Titlebook: Adult Stem Cells; Methods and Protocol Paolo Di Nardo,Sanjiv Dhingra,Vincenzo Desiderio Book 2024Latest edition The Editor(s) (if applicabl

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A Nonenzymatic Procedure to Obtain Human Mesenchymal Stromal Cells from the Dermis,earliest passages (approximately 90% positive for the classical MSC markers CD90, CD105, and CD73, while negative for the hematopoietic markers CD34 and CD45, as well as HLA-DR). Additionally, we describe the procedures for cell expansion, banking, and secretome collection.

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Mitochondrial Transfer Between Mesenchymal Stem Cells and Cancer Cells,s chapter, we outline some key protocols for evaluating this exciting phenomenon of MT. These methodological and technical approaches are very important, considering all the limitations that scientists constantly face, especially in this field of the research.

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Generation of Induced Pluripotent Stem Cells from Erythroid Progenitor Cells,SCs are incredibly valuable for . research, cell therapy, drug discovery, and tissue engineering. The outlined procedures below provide a general framework for inducing iPSCs from erythroid progenitor cells, pluripotency confirmation experiments, and cultivating them for downstream experiments.

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iPSC-Based Disease Modeling and Functional Assessment of Neurons in Patients with Metabolic Disordee chapter most importantly describes how these mature neurons can be evaluated for their activity by using multi-well microelectrode array system and its analysis. This method of generating personalized iPSC derived neurons and their endpoint assessment can be applied to many clinical and preclinica

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Generation of Directly Reprogrammed Human Endothelial Cells,d have a neovascularization capacity similar to early rECs. Both cell types can be derived from human somatic source cells, making them suitable for personalized disease investigations, drug discovery, and disease therapy.