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Titlebook: Atomic Force Microscopy; Methods and Protocol Nuno C. Santos,Filomena A. Carvalho Book 2019 Springer Science+Business Media, LLC, part of S

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期刊全称Atomic Force Microscopy
期刊简称Methods and Protocol
影响因子2023Nuno C. Santos,Filomena A. Carvalho
视频video
发行地址Includes cutting-edge lab techniques.Provides step-by-step detail essential for reproducible results.Contains key notes and advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Atomic Force Microscopy; Methods and Protocol Nuno C. Santos,Filomena A. Carvalho Book 2019 Springer Science+Business Media, LLC, part of S
影响因子.This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an .in vitro. system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Atomic Force Microscopy: Methods and Protocols. is useful for researchers at diffe
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Investigating the Nanodomain Organization of Rhodopsin in Native Membranes by Atomic Force Microscop.e., nanodomains), which facilitates their function in cell signaling processes. The visualization of membrane proteins and nanodomains within biological membranes under physiological conditions presents a challenge and is not possible using conventional microscopy methods. Atomic force microscopy (
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Analysis of DNA–Protein Complexes by Atomic Force Microscopy Imaging: The Case of TRF2–Telomeric DNAe. For 30 years, AFM has been a valuable tool in life sciences to study biological samples in the field of tissue, cellular and molecular imaging, of mechanical properties and of force spectroscopy. Since the early beginnings of the technique, AFM has been extensively exploited as an imaging tool fo
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Functionalization of AFM Tips and Supports for Molecular Recognition Force Spectroscopy and Recognitected and localized on the sample surface. Moreover, the mechanism of interaction can be studied by force spectroscopy if purified target molecules are linked to an ultra-flat surface, such as mica or silicon (nitride). Rapid imaging of the binding sites and force spectroscopy studies are greatly fa
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Single-Molecule Force Spectroscopy: Experiments, Analysis, and Simulations. The quantification of mechanical resistance of cells and biomolecules using biophysical methods matured thanks to the development of nanotechnologies such as optical and magnetic tweezers, the biomembrane force probe, and atomic force microscopy (AFM). The quantitative nature of force spectroscopy
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AFM to Study Pore-Forming Proteinse surface structure and in the force mode it can also be used to test the mechanical properties of the sample. AFM has been successfully applied to study the molecular mechanism of pore-forming proteins on model membranes. It gives information about both the structural reorganization of the membrane
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