Titlebook: Arbuscular Mycorrhizal Fungi; Methods and Protocol Nuria Ferrol,Luisa Lanfranco Book 2020 Springer Science+Business Media, LLC, part of Spr

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High-Throughput DNA Sequence-Based Analysis of AMF Communities,utrition and water supply, soil structure, and plant resistance to pathogens. Investigating AMF presence and distribution at small and large scales is critical. Therefore, research requires standard protocols to be easily implemented. In this chapter, we describe a workflow for AMF identification by

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Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymm root or soil material DNA extraction. The low taxonomic diversity of arbuscular mycorrhizal (AM) fungi makes this technique a cheap and adequate method for fingerprinting their communities. Here, I describe the TRFLP database approach, a version of the technique in which the AM fungal taxa present

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Bioinformatic Methods for the Analysis of High-Throughput RNA Sequencing in Arbuscular Mycorrhizal is focused on protein-coding RNAs, and results in large datasets of reads, or portion of sequenced mRNA that can be assembled back to the original transcripts to reconstruct a virtual gene catalog. Studies on the biology of arbuscular mycorrhizal fungi (AMF) often took great advantage of mRNA-seq,

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Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM ions during plant–microbe interactions. In this chapter, protocols to apply the LMD approach to study plant and fungal transcript profiles in different cell-type populations from arbuscular mycorrhizal (AM) roots are described in detail, starting from the biological material preparation to gene expr

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Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization,s. In particular, to capture the dynamic of the interaction, cell-specific methods for gene expression analysis are required. In situ hybridization is a technique that allows to determine the location of transcript accumulation within tissues, being of special interest for these fungi that cannot be

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Functional Analysis of Arbuscular Mycorrhizal Fungal Genes in Yeast,ason, a heterologous system has been traditionally used for functional analysis of AM fungal genes, being the budding yeast . an organism suitable for this purpose. Here we present the yeast methods required for the functional analysis of AM fungal genes, including protocols for yeast transformation

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Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycohe occupation of different niches, and dispersal of propagules, but also has strong influences upon biogeochemical cycling. By providing a valuable overview of expression changes of most proteins, shotgun proteomics can help decipher key metabolic pathways involved in the functioning of fungal mycel

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Host-Induced Gene Silencing of Arbuscular Mycorrhizal Fungal Genes via ,-Mediated Root Transformatihat are not amenable or recalcitrant to genetic modifications. This method has been particularly used for oomycetes and for filamentous fungi interacting with plants, including the fungi of the arbuscular mycorrhizal symbiosis. Here, we present a protocol developed in our laboratory to downregulate