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Titlebook: Apoptosis and Cancer; Methods and Protocol Gil Mor,Ayesha B. Alvero Book 2015Latest edition Springer Science+Business Media New York 2015 a

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发表于 2025-3-21 19:42:20 | 显示全部楼层 |阅读模式
期刊全称Apoptosis and Cancer
期刊简称Methods and Protocol
影响因子2023Gil Mor,Ayesha B. Alvero
视频video
发行地址Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
学科分类Methods in Molecular Biology
图书封面Titlebook: Apoptosis and Cancer; Methods and Protocol Gil Mor,Ayesha B. Alvero Book 2015Latest edition Springer Science+Business Media New York 2015 a
影响因子.In .Apoptosis and Cancer: Methods and Protocols., .Second Edition,. expert researches in the field detail the performance of molecular and cellular biology techniques for studying and detecting the activation of the apoptotic pathway. Chapters focus on assays developed to detect its activation not only .in vitro. but also .in vivo, .optimized multiplex analysis, medium- to high-throughput screens, and the cellular process. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Apoptosis and Cancer: Methods and Protocols, Second Edition .aids scientists as a stand-alone resource for the execution and analysis of the described protocols and as a reference for the study and detection of apoptosis within and outside the area of cancer research. .
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发表于 2025-3-21 23:40:16 | 显示全部楼层
Flow Cytometry Enumeration of Apoptotic Cancer Cells by Apoptotic Rate,I), i.e., the percentage of apoptotic cells displaying a specific linage antigen (LAg) within a population of cells that remain unfragmented and retain the expression of the LAg. However, this approach has two major limitations. Firstly, apoptotic cells fragment into apoptotic bodies that later disi
发表于 2025-3-22 03:42:38 | 显示全部楼层
,“Multiplexed Viability, Cytotoxicity, and Caspase Activity Assays”,nly more cost efficient, but also provides more information about a compound or treatment. The ability to combine the activity profiles within the same sample provides a level of normalization not possible with parallel assays. Furthermore, multiplexing caspase activity assays with viability and/or
发表于 2025-3-22 04:43:33 | 显示全部楼层
A Multiplexed Method for Kinetic Measurements of Apoptosis and Proliferation Using Live-Content Imasingle, user-defined endpoint. We describe a kinetic multiplex assay incorporating the CellPlayer. NucLight Red reagent to measure proliferation and the CellPlayer. Caspase-3/7 reagent to measure apoptosis using the two-color, live-content imaging platform, IncuCyte. ZOOM. High-definition phase-cont
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,Detection and Quantification of Apoptosis in Primary Cells Using Taqman® Protein Assay,s are used, the disadvantages of the standard methods can make apoptosis detection difficult due to their slow growth rate and replicative senescence, thereby limiting the available cell number and experiment time span. In this chapter, we describe apoptosis detection and quantification using an inn
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Detection of p53 Protein Transcriptional Activity by Chromatin Immunoprecipitation,ntegrity. An important cellular function that is dependent on p53 transcriptional activity is apoptosis or programmed cell death. Indeed, inhibition of p53 transcriptional activity is often observed in cancers as a result of mutations within its DNA-binding domain. In this chapter, we describe the u
发表于 2025-3-23 03:26:33 | 显示全部楼层
Homogeneous, Bioluminescent Proteasome Assays,proteins involved in cell-cycle control, apoptosis, and angiogenesis led to the recognition of the proteasome as a therapeutic target for cancer [1–6]. The proteasome is also essential for degrading misfolded and aberrant proteins, and impaired proteasome function has been implicated in neurodegerat
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