Titlebook: Antibiotics; Methods and Protocol Peter Sass Book 2017 Springer Science+Business Media New York 2017 antibacterial compounds.antibiotic bio

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Einführung in die Technische Mechaniktly a microorganism. In this chapter, a general workflow, which can easily be adapted to the lab, is described. Both fermentation on solid and in liquid medium is explained, and examples of hand on procedures are given.

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https://doi.org/10.1007/978-3-540-37892-1ut-driven search for novel DNA metabolism inhibitors of established and novel DNA metabolism targets in bacteria. The protocol for a colorimetric coupled primase-inorganic pyrophosphatase assay developed by our group is described in detail.

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Structure Elucidation of Antibiotics by NMR Spectroscopynal (1D) and two-dimensional (2D) NMR methods are available today and the list continues to expand. In this chapter, we will consider the key NMR experiments that provide useful information for compound structure elucidation.

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Determination of Bacterial Membrane Impairment by Antimicrobial Agents leakage of low molecular weight compounds such as ions, amino acids and ATP, and subsequent membrane depolarization. This article describes two techniques to assess antibiotic-induced membrane impairment in vivo.

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Measurement of Cell Membrane Fluidity by Laurdan GP: Fluorescence Spectroscopy and Microscopy the mechanism of action of membrane targeting antimicrobials, but the corresponding analyses have been hampered by the absence of readily available analytical tools. Here, we provide detailed protocols that allow straightforward measurement of antibiotic compound-triggered changes in membrane fluidity both in vivo and in vitro.

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