Titlebook: Animal Coronaviruses; Leyi Wang Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive license to Spring

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champaign

TaqMan Real-Time RT-PCR Detection of Porcine Epidemic Diarrhea Virusrase chain reaction (RT-PCR) using PEDV-specific primers and probe for detection and quantitation of PEDV in feces is described in this chapter. The developed TaqMan real-time RT-PCR assay is a rapid, sensitive, and reproducible detection method for PEDV and can quantitate 6.368 copies/μL of genome.

沙漠

Book 2022Latest editionown pitfalls. .Thorough and hands-on, .Animal Coronaviruses, Second Edition. will help readers design and carry out their increasingly important projects in clinical research, diagnostics, disease surveillance, and epidemiological study involving this family of viruses..

勉励

Catalina Rey Hernández,Nico Tillieiviral drugs and vaccines. In this chapter, detailed materials and methods for the cell culture isolation of PEDV variants from clinical samples are provided. Clinical sample types for the successful isolation of PEDV will be discussed.

疏远天际

Armin Fuchs,Nico N. M. J. D. Tillie,Mo Smit Vero cell culture method. The viral titer was measured by 50% tissue culture infective dose, and the virus strain was further characterized through electron microscopy, indirect immunofluorescence assay, viral replication kinetics, and sequence analysis.

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EXUDE

Isolation of Porcine Epidemic Diarrhea Virus from Clinical Samplesiviral drugs and vaccines. In this chapter, detailed materials and methods for the cell culture isolation of PEDV variants from clinical samples are provided. Clinical sample types for the successful isolation of PEDV will be discussed.

gentle

Isolation and Characterization of Porcine Epidemic Diarrhea Virus Using Suspension Vero Cells Vero cell culture method. The viral titer was measured by 50% tissue culture infective dose, and the virus strain was further characterized through electron microscopy, indirect immunofluorescence assay, viral replication kinetics, and sequence analysis.

cultivated

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