Titlebook: Animal Cell Technology: Basic & Applied Aspects; Proceedings of the F Kazumi Yagasaki,Yutaka Miura,Yoshihiro Nomura Conference proceedings

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Optimization of the Production of Retroviral Vectorsne transfer vectors. To date, a majority of clinical trials have been using vectors derived from Murine Leukemia Viruses (MLV) (Andreadis et al. 1999, ./genmed). Despite of their limitations (i.e. relatively low titer and activity, use limited to dividing cells), MLV vectors lead to permanent gene t

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Development of Apoptosis-Resistant Dihydrofolate Reductase-Deficient Chinese Hamster Ovary Cell Line in various types of cells including CHO cells. Apoptotic cell death can be triggered by several adverse conditions such as unavailability of nutrients or serum/growth factors and accumulation of toxic metabolites during cultures. Sodium butyrate (NaBu), which is widely used in rCHO cell cultures fo

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anced. The Proceedings will be useful for the cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and other disciplines related to animal cell culture, working in either academic environments or in industries of biotechnology and pharmacy.978-90-481-6557-5978-94-017-0726-8