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Titlebook: Animal Cell Culture; John M. Walker,Jeffrey W. Pollard,John M. Walker Book 1990 Springer Science+Business Media New York 1990

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发表于 2025-3-21 19:23:00 | 显示全部楼层 |阅读模式
期刊全称Animal Cell Culture
影响因子2023John M. Walker,Jeffrey W. Pollard,John M. Walker
视频video
学科分类Methods in Molecular Biology
图书封面Titlebook: Animal Cell Culture;  John M. Walker,Jeffrey W. Pollard,John M. Walker Book 1990 Springer Science+Business Media New York 1990
影响因子For many years I performed tissue culture in large scientific insti- tions that had a great deal of infrastructure. When I set up a tissue l- oratory outside such an infrastructure, however, I found there was a shortage of easily accessible information about the basic needs, reagents, and techniques for establishing such a facility. Much had to be done by trial and error or gleaned from originalpapers. Consequently, I felt that a methods book covering a wide variety of techniques from basic culture to the most sophisticated cell analysis would be a very valuable addition to the scientific literature. In the interim, several useful books (listed in Chapter I of this volume) did appear, but none entirely fitted the bill and some are now somewhat dated. Then, in 1984, the first of the Methods in Molecular Biology volumes from Humana Press was published with its step-by-step recipe approach. This format appealed to me, and so I c- tacted John Walker, the series editor, about including cell culture in this series. The result was that we embarked upon a single volume covering both plant and animal cell culture. Such was the richness of the material that this project soon divided itself i
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Eating Characteristics and Temperamentich is passed through the abdominal wall. In both these methods, the positioning of the aspirating device must be made with the help of ultrasound scanning. Later pregnancies can only be sampled by the transabdominal route. Amniocentesis is usually performed at 16–18 wk of gestation by the transabdominal method.
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Short-Term Chorionic Villi and Amniotic Fluid Cultures,ich is passed through the abdominal wall. In both these methods, the positioning of the aspirating device must be made with the help of ultrasound scanning. Later pregnancies can only be sampled by the transabdominal route. Amniocentesis is usually performed at 16–18 wk of gestation by the transabdominal method.
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Embryonic Rodent Brain Cells in Culture,ed conditions. Methods are available now to isolate and grow individual classes of neural cells (i.e., neurons, astrocytes, oligodendrocytes), thereby enabling one to study the cellular behavior at the individual level and to uncover the nature of cell-cell interactions that presumably govern cell differentiation.
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Basic Cell Culture,ransformed derivatives, and the isolation of genetically pure strains. The cells described are all derived from Chinese hamsters since, to date, these cells, have proved to be the most useful for somatic cell genetics (.,.). The techniques, however, are generally applicable to most fibroblastic cell types.
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