Titlebook: Analysis of Post-Translational Modifications and Proteolysis in Neuroscience; Jennifer Elizabeth Grant,Hong Li Book 2016 Springer Science+

Bravura

Mass Spectrometric Detection of Detyrosination and Polyglutamylation on the C-Terminal Region of Brtry (MS) is a powerful approach. In this chapter, we provide a convenient procedure specialized for polyglutamylation of β-tubulin as well as a conventional procedure for all three modifications of both α- and β-tubulin.

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杀人

,III. The conjugate space of Φ,lation, succinylation, ubiquitination, and cleaved caspase proteolytic processing. Immunoaffinity LC-MS has been routinely used for characterizing changes in response to a multitude of biological perturbations or treatment conditions, and is applicable for numerous model systems and species whose ge

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A. Bohm,J. D. Dollard,M. Gadellaides are removed. By virtue of the universal boronic acid-diol recognition, large-scale mapping of N-glycoproteins can be achieved by combining boronic acid-based enrichment, PNGase F treatment in the presence of heavy oxygen (.O) water, and MS analysis. This method can be extensively applied for th

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https://doi.org/10.1007/978-0-8176-4493-2e cysteome, we developed a mass spectrometry-based approach, denoted as d-SSwitch, using isotopic labeling and shotgun proteomics to simultaneously identify and quantify different modification states at individual cysteine residues. Both recombinant protein and intact neuroblastoma cells were analyz

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Dirac Operators in the Algebraic Setting,onse to hydrogen peroxide is conserved in mouse hippocampal HT22 cells, highlighting the importance of this modification in higher eukaryotes. In this chapter, we discuss cutting-edge methodologies available to investigate protein ubiquitination in a proteome-wide and quantitative manner, and we pre

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Dirac Operators in the Algebraic Setting,growth factor (NGF), after a period of 1–2 min. The identification of the modified residue and its location in the sequence of the peptide can be determined following collision induced dissociation of the precursor peptide in the mass spectrometer and the observation of fragment ions that frame the

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