Titlebook: Alzheimer’s Disease; Methods and Protocol Jerold Chun Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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https://doi.org/10.1007/978-3-322-98858-4es. Culturing human primary cortical astrocyte (pHA)- or induced pluripotent stem cell (iPSC)-derived human neural stem cells in this biohybrid hydrogel system has led to the discovery of novel regulatory pathways underlying neurodegenerative pathology in different phases of AD.

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,Implizites Wissen und Innovationsprozeß,e harmonization of results across independent studies. In this chapter, we contextualize these issues with recent reports identifying correlations between brain lipids and neurological diseases, and we describe the workflow our group has optimized for analysis of the blood plasma sphingolipidome, ad

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,Implizites Wissen und Innovationsprozeß, spectrometer to scan brain tissue slices with high spatial resolution, analyzing 50 μm cell layers. The lipid spectra obtained for each pixel are transformed to color-coded intensity maps of hundreds of lipid species included those within a single tissue slice.

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Mass Spectrometry for the Advancement of Lipid Analysis in Alzheimer’s Research spectrometer to scan brain tissue slices with high spatial resolution, analyzing 50 μm cell layers. The lipid spectra obtained for each pixel are transformed to color-coded intensity maps of hundreds of lipid species included those within a single tissue slice.