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Titlebook: Alkaline Phosphatase; Robert B. McComb,George N. Bowers,Solomon Posen Book 1979 Plenum Press, New York 1979 Amino acid.bacteria.biochemist

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Die Verkehrsbedienung im Personennahverkehr,In this chapter, we discuss the estimation of alkaline phosphatase in blood serum and other biological fluids of interest to veterinarians. Tissue alkaline phosphatases are discussed in Chapter 3.
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Distribution in Nature,Alkaline phosphatases are widely distributed in nature. There are considerable species-and organ-related differences; even within the same organ and the same cell, enzymatic activity is likely to be unevenly distributed. Changes have been reported in relation to growth and development and to cylical events such as estrus.
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Structural Features,In this chapter, we deal with the structural features of alkaline phosphatases from different sources. We describe common characteristics such as metal and carbohydrate content, amino acid analysis, and, where available, amino acid sequences. Aggregation and disaggregation of subunits and molecular-weight measurements are also discussed.
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Reaction Mechanisms,In this chapter, we first describe some of the compounds of phosphorus that can serve as substrates in the alkaline phosphatase reaction. We discuss some of their chemical characteristics, and their nonenzymatic hydrolysis. We then go on to examine the sequence of the alkaline phosphatase reaction step by step.
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Measurement of Alkaline Phosphatase Activity,This chapter will discuss some practical aspects of alkaline phosphatase assays. While the remarks are based predominantly on experience gained with human blood serum, many of the points raised are also applicable to the assay of alkaline phosphatases from other sources.
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Utilization of Alkaline Phosphatase Measurements in Veterinary Practice,In this chapter, we discuss the estimation of alkaline phosphatase in blood serum and other biological fluids of interest to veterinarians. Tissue alkaline phosphatases are discussed in Chapter 3.
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