Titlebook: Agrobacterium Protocols; Volume I Kan Wang Book 2006Latest edition Humana Press 2006 Agrobacterium tumefaciens.Arabidopsis thaliana.DNA.Flo

left-ventricle

(,)for rapid evaluation of transgenes in higher plants. This protocol has a number of desirable attributes: readily available explant material, high efficiency, and a relatively quick turnaround time. The in vitro regeneration scheme of the leaf disks is prolific and follows an indirect organogenic dif

Inferior

粘

Barley (, L.)would be of great value. Barley has been transformed using microprojectile bombardment and by direct gene transfer to protoplasts, but neither method has been able to produce fertile transformants in large numbers with simple transgene integration characteristics. .-mediated transformation was first

钱财

Maize (, L.)ormation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an . strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plan

Callus

micronutrients

主动

在前面

恩惠

fender

https://doi.org/10.1007/978-3-031-44093-9ther electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into . using the triparental mating method. Here we present three detailed protocols for . strain construction using electroporation, the freeze/thaw method of transformation, and triparental mating.