期刊全称 | Advances in GAPDH Protein Analysis: A Functional and Biochemical Approach | 影响因子2023 | Shanmugasundaram Ganapathy-Kanniappan | 视频video | | 发行地址 | Is a unique compendium that offers a complete solution of protocols on GAPDH protein analysis for researchers at all levels including undergraduates, graduates,postgraduates and research scientists in | 图书封面 |  | 影响因子 | .This book presents modern and classic analytical approaches that are crucial for the biochemical and functional characterization of the archetypal protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The distinguishing feature of the book is that it covers, in addition to other methods, some of the uncommon but valuable techniques as well. For example, in-gel visualization of enzyme activity, immunoblotting protocols for native (non-denatured) proteins, and proteins resolved by pH-gradient [IEF-isoelectrofocusing], etc. These expedient methods are relevant and vital for the verification of biochemical properties of GAPDH, or similar protein of interest. ..This work outlines detailed protocols that are essential to investigate classical (cellular) and recently reported extracellular (secretory) isoforms of GAPDH. Precisely, the book covers techniques pertinent to enzymatic and non-enzymatic analysis of GAPDH that include, but not limited to, electrophoretic mobility shift assay (EMSA), two-dimensional (2D)-immunoblotting, immunofluorescence/confocal microscopy, mass spectrometry, ion-exchange and affinity chromatography. ..Readers will discover the importance of the experime | Pindex | Book 2017 |
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