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Titlebook: Advances in GAPDH Protein Analysis: A Functional and Biochemical Approach; Shanmugasundaram Ganapathy-Kanniappan Book 2017 Springer Nature

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发表于 2025-3-21 19:51:11 | 显示全部楼层 |阅读模式
期刊全称Advances in GAPDH Protein Analysis: A Functional and Biochemical Approach
影响因子2023Shanmugasundaram Ganapathy-Kanniappan
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发行地址Is a unique compendium that offers a complete solution of protocols on GAPDH protein analysis for researchers at all levels including undergraduates, graduates,postgraduates and research scientists in
图书封面Titlebook: Advances in GAPDH Protein Analysis: A Functional and Biochemical Approach;  Shanmugasundaram Ganapathy-Kanniappan Book 2017 Springer Nature
影响因子.This book presents modern and classic analytical approaches that are crucial for the biochemical and functional characterization of the archetypal protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The distinguishing feature of the book is that it covers, in addition to other methods, some of the uncommon but valuable techniques as well. For example, in-gel visualization of enzyme activity, immunoblotting protocols for native (non-denatured) proteins, and proteins resolved by pH-gradient [IEF-isoelectrofocusing], etc. These expedient methods are relevant and vital for the verification of biochemical properties of GAPDH, or similar protein of interest. ..This work outlines detailed protocols that are essential to investigate classical (cellular) and recently reported extracellular (secretory) isoforms of GAPDH. Precisely, the book covers techniques pertinent to enzymatic and non-enzymatic analysis of GAPDH that include, but not limited to, electrophoretic mobility shift assay (EMSA), two-dimensional (2D)-immunoblotting, immunofluorescence/confocal microscopy, mass spectrometry, ion-exchange and affinity chromatography.  ..Readers will discover the importance of the experime
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发表于 2025-3-21 23:07:27 | 显示全部楼层
https://doi.org/10.1007/978-3-540-49150-7eviously described protocols, the emphasis here will be on methods that require detailed, special procedures for detection of specific modifications such as oxidation, aggregation and .-GlcNAcylation.
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发表于 2025-3-22 11:10:43 | 显示全部楼层
The Management of Laboratory Information,s this offers very robust validation and avoids the need for additional steps in signal detection. Although, recent advances provide the option of using nonradioactive probes, the selection and choice of probe labeling highly depends upon the user and the nature of the analysis.
发表于 2025-3-22 15:19:10 | 显示全部楼层
Experimental Design and Optimization,APDH. The scope of this chapter is to provide recent advances rather than a review of literature. Hence the emphasis will be on significant advancements in current protocols. The method described here in for purification is based on the recent identification of novel isoform of GAPDH which is a high molecular weight, secretory GAPDH.
发表于 2025-3-22 17:50:26 | 显示全部楼层
Method Validation and Laboratory Evaluation,is will be placed on the details of isolation and preparation of GAPDH from respective cellular compartments. Technical details of the procedures involved in the immunodetection by immunoblotting have been discussed in the Chap. ..
发表于 2025-3-22 23:30:55 | 显示全部楼层
Experimental Design and Optimization,will outline current protocols that are used and relevant in the identification of peptides of GAPDH. Particularly, the protocol, liquid chromatography–tandem mass spectrometry (LC–MS/MS) which has been used and validated in our laboratory for GAPDH identification will be discussed in detail.
发表于 2025-3-23 04:17:05 | 显示全部楼层
Shanmugasundaram Ganapathy-KanniappanIs a unique compendium that offers a complete solution of protocols on GAPDH protein analysis for researchers at all levels including undergraduates, graduates,postgraduates and research scientists in
发表于 2025-3-23 08:34:46 | 显示全部楼层
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