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Titlebook: Advanced Technologies for Protein Complex Production and Characterization; Volume II M. Cristina Vega,Francisco J. Fernández Book 2024 The

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发表于 2025-3-21 17:24:44 | 显示全部楼层 |阅读模式
期刊全称Advanced Technologies for Protein Complex Production and Characterization
期刊简称Volume II
影响因子2023M. Cristina Vega,Francisco J. Fernández
视频video
发行地址Useful companion to the first volume on Protein Complex Production.Focuses on the characterization of protein complexes.Written by renowned active scientists in the field
学科分类Advances in Experimental Medicine and Biology
图书封面Titlebook: Advanced Technologies for Protein Complex Production and Characterization; Volume II M. Cristina Vega,Francisco J. Fernández Book 2024 The
影响因子.This edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume.. .The readers will find an integral connection between this book and the first volume, which ensures a comprehensive and updated discussion of the main topics of the discipline. Taken together, these volumes constitute a cohesive and authoritative source of the research on multicomponent macromolecular
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Geltung der Grundrechte vor der Geburt will review experimental and . techniques that can be used for the discovery and characterization of linear motif mediated protein-protein complexes involved in cellular signaling, protein level and gene expression regulation.
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Private Health Care, Quality and Corruption,isolate RNPs. This chapter describes different approaches and methods devised for RNA-specific purification of a target RNP. We focused on general methods for selecting RNPs that target a given RNA under conditions favourable for the copurification of associated factors including RNAs and protein components of the RNP.
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,BME in der Europäischen Gemeinschaft,e, and the method of solubilization from the membrane for membrane proteins. It further depends on the choice of sample support, grid pre-treatment and cryo-grid freezing protocol. Here, we discuss various widely applicable approaches to improve sample quality for structural analysis by cryo-EM.
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Embryonen- und Stammzellforschungese techniques as orthogonal analytical methods to analyze protein oligomerization and polymerization, interactions with ligands, hetero-associations involving membrane proteins, and protein-nucleic acid complexes.
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