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Titlebook: Advanced Photon Counting; Applications, Method Peter Kapusta,Michael Wahl,Rainer Erdmann Book 2015 Springer International Publishing Switze

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期刊全称Advanced Photon Counting
期刊简称Applications, Method
影响因子2023Peter Kapusta,Michael Wahl,Rainer Erdmann
视频video
发行地址A comprehensive review.Written by experts.Richly illustrated.Includes supplementary material:
学科分类Springer Series on Fluorescence
图书封面Titlebook: Advanced Photon Counting; Applications, Method Peter Kapusta,Michael Wahl,Rainer Erdmann Book 2015 Springer International Publishing Switze
影响因子.This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluct
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https://doi.org/10.1007/978-3-030-63982-2t pulse width (usually picoseconds or femtoseconds) as well as repetition rates in the kilohertz to megahertz range. The repetition rate should ideally be tunable in order to adapt the pulse period to the required measurement window. In the blue, red, and IR spectral range, such pulses with energies
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https://doi.org/10.1007/978-3-030-63982-2al-focus FCS (2fFCS). We decided to put our focus on a detailed discussion of these two – and in our eyes well-merited – advanced methods, rather than giving an overview over the broad variety of advanced FCS methods that would consequently lack detail and leave the reader rather uneducated on all t
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https://doi.org/10.1007/978-3-319-24987-2nt spectral properties. PIE was originally introduced to enable artifact-free fluorescence cross-correlation measurements, while first experiments with alternating laser excitation (ALEX) used the dual excitation of donor and acceptor for single-pair Förster resonance energy transfer (spFRET). In th
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